小柯机器人

以色列理工学院Ruby Shalom-Feuerstein和Yonatan Savir研究组合作取得最新进展。他们发现离散的角膜边缘上皮干细胞（LSC）群体介导角膜稳态和伤口愈合。2021年5月12日出版的《细胞-干细胞》杂志发表了这一成果。

他们结合了单细胞RNA测序和先进的定量谱系追踪技术，以深入分析鼠的角膜缘上皮细胞。这些分析表明，两个LSC群体共存于独立且定义明确的亚区域，称为“外”和“内”角膜缘。静息的外部LSC的原始群体参与伤口愈合和边界形成，并且这些细胞受T细胞的调节，而T细胞是一种微环境。

相比之下，内侧角膜周围角膜缘拥有活跃的LSC，这些LSC维持角膜上皮稳态。定量分析表明，遵循随机规则和中性漂移动态，LSC群体非常丰富。这些结果共同表明，离散的LSC群体介导角膜稳态和再生。

据了解，角膜的可及性和透明性可实现可靠的干细胞标记和体内细胞命运定位。传统上，更新罕见的、循环缓慢的LSC，遵循确定性规则指示其自我更新或分化。

附：英文原文

Title: Discrete limbal epithelial stem cell populations mediate corneal homeostasis and wound healing

Author: Anna Altshuler, Aya Amitai-Lange, Noam Tarazi, Sunanda Dey, Lior Strinkovsky, Shira Hadad-Porat, Swarnabh Bhattacharya, Waseem Nasser, Jusuf Imeri, Gil Ben-David, Ghada Abboud-Jarrous, Beatrice Tiosano, Eran Berkowitz, Nathan Karin, Yonatan Savir, Ruby Shalom-Feuerstein

Issue&Volume: 2021-05-12

Abstract: The accessibility and transparency of the cornea permit robust stem cell labelingand in vivo cell fate mapping. Limbal epithelial stem cells (LSCs) that renew the cornea aretraditionally viewed as rare, slow-cycling cells that follow deterministic rules dictatingtheir self-renewal or differentiation. Here, we combined single-cell RNA sequencingand advanced quantitative lineage tracing for in-depth analysis of the murine limbalepithelium. These analysis revealed the co-existence of two LSC populations localizedin separate and well-defined sub-compartments, termed the “outer” and “inner” limbus.The primitive population of quiescent outer LSCs participates in wound healing andboundary formation, and these cells are regulated by T cells, which serve as a niche.In contrast, the inner peri-corneal limbus hosts active LSCs that maintain cornealepithelial homeostasis. Quantitative analyses suggest that LSC populations are abundant,following stochastic rules and neutral drift dynamics. Together these results demonstratethat discrete LSC populations mediate corneal homeostasis and regeneration.

DOI: 10.1016/j.stem.2021.04.003

Source: https://www.cell.com/cell-stem-cell/fulltext/S1934-5909(21)00160-0

Cell Stem Cell：《细胞—干细胞》，创刊于2007年。隶属于细胞出版社，最新IF：25.269
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