小柯机器人

四川大学华西第二医院郭帆和王平团队合作取得最新进展。他们使用单细胞多组学测序对人卵母细胞中动态表观遗传学景观进行解码。该研究于2021年5月5日发表于国际一流学术期刊《细胞-干细胞》杂志上。

他们开发了一种单细胞多组学测序方法scChaRM-seq，该方法可以在单个人卵母细胞和卵巢体细胞中同时分析DNA甲基化组、转录组和染色质可及性。他们观察到人类卵母细胞生长期间DNA甲基化的整体增加与染色质可及性相关，而特定特征处DNA甲基化的增加与活性转录相关。

对来自人类和小鼠的多组学数据的综合分析显示了物种特异性基因表达，启动子可及性有助于基因区域甲基化程序。Alu元素在早期生长的卵母细胞中保持较低的DNA甲基化水平和较高的可及性，并且位于人和小鼠的发育基因附近。这些发现共同表明scChaRM-seq如何提供对DNA甲基化模式建立的见解。

据介绍，发育中的女性人类生殖细胞经历了全基因组的表观遗传重编程，但人们对从头DNA甲基化动力学及其与染色质状态的相互作用以及发育中的卵母细胞中的转录激活了解甚少。

附：英文原文

Title: Decoding dynamic epigenetic landscapes in human oocytes using single-cell multi-omics sequencing

Author: Rui Yan, Chan Gu, Di You, Zhongying Huang, Jingjing Qian, Qiuyun Yang, Xin Cheng, Lin Zhang, Hongmei Wang, Ping Wang, Fan Guo

Issue&Volume: 2021-05-05

Abstract: Developing female human germ cells undergo genome-wide epigenetic reprogramming, butde novo DNA methylation dynamics and their interplay with chromatin states and transcriptionalactivation in developing oocytes is poorly understood. Here, we developed a single-cellmulti-omics sequencing method, scChaRM-seq, that enables simultaneous profiling ofthe DNA methylome, transcriptome, and chromatin accessibility in single human oocytesand ovarian somatic cells. We observed a global increase in DNA methylation duringhuman oocyte growth that correlates with chromatin accessibility, whereas increasesof DNA methylation at specific features were associated with active transcription.Integrated analyses of multi-omics data from humans and mice revealed species-specificgene expression, and promoter accessibility contributes to gene body methylation programs.Alu elements retained low DNA methylation levels and high accessibility in early growingoocytes and were located near developmental genes in humans and mice. Together, thesefindings show how scChaRM-seq can provide insight into DNA methylation pattern establishment.

DOI: 10.1016/j.stem.2021.04.012

Source: https://www.cell.com/cell-stem-cell/fulltext/S1934-5909(21)00169-7

Cell Stem Cell：《细胞—干细胞》，创刊于2007年。隶属于细胞出版社，最新IF：25.269
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