荷兰皇家艺术与科学院Hans Clevers团队利用患者来源的类器官对宫颈癌的宫颈组织动态和病毒致癌作用进行了建模。这一研究成果于2021年4月13日在线发表在国际学术期刊《细胞—干细胞》上。
研究人员报道了宫颈外皮和宫颈上皮的长期培养方案，该方案可生成能够稳定模拟两个起源组织的3D类器官。正如HSV-1所证明的那样，基于类器官的宫颈模型可能有助于研究性传播导致的感染。从巴氏刷的材料开始，研究人员建立了一个由受影响个体衍生的小型类瘤生物库，其保留了致病性人乳头瘤病毒（HPV）基因组。
这些其中之一独特地携带了表征较少的HPV30亚型，这暗示了其在致癌作用中的潜在作用。类瘤表现出对常见化学治疗剂的不同反应，并在小鼠中以异种移植的形式生长。这项研究描述了一个用于宫颈（癌症）研究和未来个性化医学方法的实验平台。
据悉，宫颈癌是常见的妇科恶性肿瘤，通常由高危的人乳头瘤病毒引起。目前缺乏人类来源的培养系统来研究宫颈上皮及其衍生的癌症。
附：英文原文
Title: Patient-derived organoids model cervical tissue dynamics and viral oncogenesis in cervical cancer
Author: Kadi Lhmussaar, Rurika Oka, Jose Espejo Valle-Inclan, Milou H.H. Smits, Hila Wardak, Jeroen Korving, Harry Begthel, Natalie Proost, Marieke van de Ven, Onno W. Kranenburg, Trudy G.N. Jonges, Ronald P. Zweemer, Sebastiaan Veersema, Ruben van Boxtel, Hans Clevers
Issue&Volume: 2021-04-13
Abstract: Cervical cancer is a common gynecological malignancy often caused by high-risk humanpapillomavirus. There is a paucity of human-derived culture systems to study the cervicalepithelium and the cancers derived thereof. Here we describe a long-term culturingprotocol for ecto- and endocervical epithelia that generates 3D organoids that stablyrecapitulate the two tissues of origin. As evidenced for HSV-1, organoid-based cervicalmodels may serve to study sexually transmitted infections. Starting from Pap brushmaterial, a small biobank of tumoroids derived from affected individuals was establishedthat retained the causative human papillomavirus (HPV) genomes. One of these uniquelycarried the poorly characterized HPV30 subtype, implying a potential role in carcinogenesis.The tumoroids displayed differential responses to common chemotherapeutic agents andgrew as xenografts in mice. This study describes an experimental platform for cervical(cancer) research and for future personalized medicine approaches.
DOI: 10.1016/j.stem.2021.03.012
Source: https://www.cell.com/cell-stem-cell/fulltext/S1934-5909(21)00118-1
Cell Stem Cell：《细胞—干细胞》，创刊于2007年。隶属于细胞出版社，最新IF：25.269
官方网址：https://www.cell.com/cell-stem-cell/home
投稿链接：https://www.editorialmanager.com/cell-stem-cell/default.aspx
本期文章：《细胞—干细胞》：Online/在线发表
