小柯机器人

日本国立自然科学研究院Shosei Yoshida和英国剑桥大学Benjamin D. Simons研究团队合作取得最新进展。他们发现短暂抑制移植的精原干细胞分化可恢复小鼠的生育能力。2021年4月12日出版的《细胞-干细胞》杂志发表了这项成果。

他们对宿主生精小管中移植的小鼠精原干细胞进行了定量克隆命运研究。他们发现，在大量供体精原细胞定居于宿主睾丸后，通过随机的命运选择，只有一小部分长期存在并再生，其余的则因分化和细胞死亡而丢失。此外，基于这些见解，他们显示了如何通过使用视黄酸合成的化学抑制剂暂时抑制分化，将繁殖效率提高到不育宿主的生育力得以恢复的水平。这些发现释放了精子移植的一系列潜在应用，如从恢复癌症个体的生育能力到保护生物多样性。

据介绍，组织干细胞的显著特征是它们在移植后能够再生宿主组织的结构和功能的能力。然而，供体干细胞在再生过程中的动力学仍然很大程度上未知。

附：英文原文

Title: Transient suppression of transplanted spermatogonial stem cell differentiation restores fertility in mice

Author: Yoshiaki Nakamura, David J. Jrg, Yayoi Kon, Benjamin D. Simons, Shosei Yoshida

Issue&Volume: 2021-04-12

Abstract: A remarkable feature of tissue stem cells is their ability to regenerate the structure and function of host tissue following transplantation. However, the dynamics of donor stem cells during regeneration remains largely unknown. Here we conducted quantitative clonal fate studies of transplanted mouse spermatogonial stem cells in host seminiferous tubules. We found that, after a large population of donor spermatogonia settle in host testes, through stochastic fate choice, only a small fraction persist and regenerate over the long term, and the rest are lost through differentiation and cell death. Further, based on these insights, we showed how repopulation efficiency can be increased to a level where the fertility of infertile hosts is restored by transiently suppressing differentiation using a chemical inhibitor of retinoic acid synthesis. These findings unlock a range of potential applications of spermatogonial transplantation, from fertility restoration in individuals with cancer to conservation of biological diversity.

DOI: 10.1016/j.stem.2021.03.016

Source: https://www.cell.com/cell-stem-cell/fulltext/S1934-5909(21)00122-3

Cell Stem Cell：《细胞—干细胞》，创刊于2007年。隶属于细胞出版社，最新IF：25.269
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