意大利那不勒斯大学Antonio Randazzo团队揭示了序列和碱基组成对i-DNA的CD和TDS谱的影响。 相关研究成果于2021年2月22日发表在《德国应用化学》。
i-motif DNA，也称为i-DNA，是一种由富含胞嘧啶的序列形成的非典型DNA二级结构，由半质子化的胞嘧啶(C:C + + )碱基对连接在一起的两个插入的平行双链组成。随着人们对i‐DNA结构作为抗癌治疗靶点的兴趣日益浓厚，人们越来越需要能够快速而有意义地解释i‐DNA样品光谱数据的工具。
该文采用多元数据分析的方法，分析了255个DNA序列的圆二色性（CD）和热差紫外吸收光谱（TDS），旨在揭示在DNA形成序列分析中可作为诊断特征的特殊光谱区域。
附：英文原文
Title: Effects of sequence and base composition on the CD and TDS profiles of i‐DNA
Author: Nunzia Iaccarino, Mingpan Cheng, Dehui Qiu, Bruno Pagano, Jussara Amato, Anna Di Porzio, Jun Zhou, Antonio Randazzo, Jean-Louis Mergny
Issue&Volume: 2021-02-22
Abstract: The i‐motif DNA, also known as i‐DNA, is a non‐canonical DNA secondary structure formed by cytosine‐rich sequences, consisting of two intercalated parallel‐stranded duplexes held together by hemi‐protonated cytosine‐cytosine + (C:C + ) base pairs. The growing interest for the i‐DNA structure as a target in anticancer therapy increases the need for tools allowing a rapid and meaningful interpretation of the spectroscopic data of i‐DNA samples. Herein, we analyzed the circular dichroism (CD) and thermal difference UV‐absorbance spectra (TDS) of 255 DNA sequences by means of multivariate data analysis, aiming at unveiling peculiar spectral regions that could be used as diagnostic features during the analysis of i‐DNA‐forming sequences.
DOI: 10.1002/anie.202016822
Source: https://onlinelibrary.wiley.com/doi/10.1002/anie.202016822
