小柯机器人

室旁下丘脑细胞类群的分子组成决定行为状态
2020-10-17 23:01

室旁下丘脑细胞类群的分子组成决定行为状态,这一成果由美国霍华德·休斯医学研究所 Scott M. Sternson团队经过不懈努力而取得。相关论文于2020年10月16日发表在《科学》杂志上。

利用基于记录体内单神经元钙离子动力学附加基因表达分析的方法,研究人员开发了CaRMA(钙和RNA多重活性)成像。利用该系统,研究人员可同时监测了小鼠脑室下丘脑(PVH)中数百个神经元的活动。组合细胞特异性标记基因可预测11种行为状态下神经元反应的能力。

PVH运用组装分子合集确定了对生存行为进行分组编码的神经元类群。神经肽受体1的Y受体(Npy1r)合并具有相似反应的多种细胞类型。该研究结果表明,分子特异的神经元是大脑功能的重要组成。

据了解,大脑利用由基因表达进行系统分类的神经元来调控行为。由于难以测量同一细胞内的神经动力学和分子信息,因此无法理解分子同一性对神经编码的贡献。

附:英文原文

Title: Behavioral state coding by molecularly defined paraventricular hypothalamic cell type ensembles

Author: Shengjin Xu, Hui Yang, Vilas Menon, Andrew L. Lemire, Lihua Wang, Fredrick E. Henry, Srinivas C. Turaga, Scott M. Sternson

Issue&Volume: 2020/10/16

Abstract: Brains encode behaviors using neurons amenable to systematic classification by gene expression. The contribution of molecular identity to neural coding is not understood because of the challenges involved with measuring neural dynamics and molecular information from the same cells. We developed CaRMA (calcium and RNA multiplexed activity) imaging based on recording in vivo single-neuron calcium dynamics followed by gene expression analysis. We simultaneously monitored activity in hundreds of neurons in mouse paraventricular hypothalamus (PVH). Combinations of cell-type marker genes had predictive power for neuronal responses across 11 behavioral states. The PVH uses combinatorial assemblies of molecularly defined neuron populations for grouped-ensemble coding of survival behaviors. The neuropeptide receptor neuropeptide Y receptor type 1 (Npy1r) amalgamated multiple cell types with similar responses. Our results show that molecularly defined neurons are important processing units for brain function.

DOI: 10.1126/science.abb2494

Source: https://science.sciencemag.org/content/370/6514/eabb2494

Science:《科学》,创刊于1880年。隶属于美国科学促进会,最新IF:41.037
官方网址:https://www.sciencemag.org/
投稿链接:https://cts.sciencemag.org/scc/#/login

本期文章:《科学》:Volume 370 Issue 6514

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