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宿主与冠状病毒的蛋白相互作用网络揭示泛病毒疾病机理
2020-10-17 23:10

美国加州大学旧金山分校Nevan J. Krogan等研究人员合作利用宿主与冠状病毒蛋白相互作用网络,揭示出泛病毒疾病机理。相关论文于2020年10月15日在线发表在《科学》杂志上。

据研究人员介绍,由SARS-CoV-2冠状病毒引起的COVID-19大流行是对公共卫生和全球经济的重大威胁。SARS-CoV-2与致死性更强、更不易传播的冠状病毒SARS-CoV-1和MERS-CoV密切相关。

研究人员对所有三种病毒进行了病毒与人类的蛋白质-蛋白质相互作用以及病毒蛋白质定位分析。随后的功能遗传筛选确定了在功能上影响冠状病毒增殖的宿主因子,包括Tom70(一种线粒体伴侣蛋白,可与SARS-CoV-1和SARS-CoV-2 Orf9b相互作用),研究人员使用cryo-EM在结构上表征了这种相互作用。 将经过基因验证的宿主因素与COVID-19患者的遗传数据和医疗账单记录相结合,可以确定重要的分子机制和潜在的药物治疗方法,这值得进一步的分子和临床研究。

附:英文原文

Title: Comparative host-coronavirus protein interaction networks reveal pan-viral disease mechanisms

Author: David E. Gordon, Joseph Hiatt, Mehdi Bouhaddou, Veronica V. Rezelj, Svenja Ulferts, Hannes Braberg, Alexander S. Jureka, Kirsten Obernier, Jeffrey Z. Guo, Jyoti Batra, Robyn M. Kaake, Andrew R. Weckstein, Tristan W. Owens, Meghna Gupta, Sergei Pourmal, Erron W. Titus, Merve Cakir, Margaret Soucheray, Michael McGregor, Zeynep Cakir, Gwendolyn Jang, Matthew J. O’Meara, Tia A. Tummino, Ziyang Zhang, Helene Foussard, Ajda Rojc, Yuan Zhou, Dmitry Kuchenov, Ruth Hüttenhain, Jiewei Xu

Issue&Volume: 2020/10/15

Abstract: AbstractThe COVID-19 (Coronavirus disease-2019) pandemic, caused by the SARS-CoV-2 coronavirus, is a significant threat to public health and the global economy. SARS-CoV-2 is closely related to the more lethal but less transmissible coronaviruses SARS-CoV-1 and MERS-CoV. Here, we have carried out comparative viral-human protein-protein interaction and viral protein localization analysis for all three viruses. Subsequent functional genetic screening identified host factors that functionally impinge on coronavirus proliferation, including Tom70, a mitochondrial chaperone protein that interacts with both SARS-CoV-1 and SARS-CoV-2 Orf9b, an interaction we structurally characterized using cryo-EM. Combining genetically-validated host factors with both COVID-19 patient genetic data and medical billing records identified important molecular mechanisms and potential drug treatments that merit further molecular and clinical study.

DOI: 10.1126/science.abe9403

Source: https://science.sciencemag.org/content/early/2020/10/14/science.abe9403

Science:《科学》,创刊于1880年。隶属于美国科学促进会,最新IF:41.037
官方网址:https://www.sciencemag.org/
投稿链接:https://cts.sciencemag.org/scc/#/login

本期文章:《科学》:Online/在线发表

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