小柯机器人

近日，美国普林斯顿大学Joshua D. Rabinowitz、Sheng Hui等研究人员合作开发出循环代谢物通量的定量检测方法。2020年8月12日，《细胞—代谢》在线发表了这一成果。

Title: Quantitative Fluxomics of Circulating Metabolites

Author: Sheng Hui, Alexis J. Cowan, Xianfeng Zeng, Lifeng Yang, Tara TeSlaa, Xiaoxuan Li, Caroline Bartman, Zhaoyue Zhang, Cholsoon Jang, Lin Wang, Wenyun Lu, Jennifer Rojas, Joseph Baur, Joshua D. Rabinowitz

Issue&Volume: 2020-08-12

Abstract: Mammalian organs are nourished by nutrients carried by the blood circulation. Thesenutrients originate from diet and internal stores, and can undergo various interconversionsbefore their eventual use as tissue fuel. Here we develop isotope tracing, mass spectrometry,and mathematical analysis methods to determine the direct sources of circulating nutrients,their interconversion rates, and eventual tissue-specific contributions to TCA cyclemetabolism. Experiments with fifteen nutrient tracers enabled extensive accountingfor both circulatory metabolic cycles and tissue TCA inputs, across fed and fastedmice on either high-carbohydrate or ketogenic diet. We find that a majority of circulatingcarbon flux is carried by two major cycles: glucose-lactate and triglyceride-glycerol-fattyacid. Futile cycling through these pathways is prominent when dietary content of theassociated nutrients is low, rendering internal metabolic activity robust to foodchoice. The presented in vivo flux quantification methods are broadly applicable to different physiological anddisease states.

DOI: 10.1016/j.cmet.2020.07.013

Source: https://www.cell.com/cell-metabolism/fulltext/S1550-4131(20)30371-5

Cell Metabolism：《细胞—代谢》，创刊于2005年。隶属于细胞出版社，最新IF：31.373
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