小柯机器人

美国波士顿大学医学院Mikel Garcia-Marcos研究组利用多功能生物传感器揭示了活细胞中三聚体G蛋白的活性。相关论文于2020年7月6日在线发表在《细胞》杂志上。

Title: Revealing the Activity of Trimeric G-proteins in Live Cells with a Versatile Biosensor Design

Author: Marcin Maziarz, Jong-Chan Park, Anthony Leyme, Arthur Marivin, Alberto Garcia-Lopez, Prachi P. Patel, Mikel Garcia-Marcos

Issue&Volume: 2020-07-06

Abstract: Heterotrimeric G-proteins (Gαβγ) are the main transducers of signals from GPCRs, mediating the action of countless natural stimuli and therapeutic agents. However, there are currently no robust approaches to directly measure the activity of endogenous G-proteins in cells. Here, we describe a suite of optical biosensors that detect endogenous active G-proteins with sub-second resolution in live cells. Using a modular design principle, we developed genetically encoded, unimolecular biosensors for endogenous Gα-GTP and free Gβγ: the two active species of heterotrimeric G-proteins. This design was leveraged to generate biosensors with specificity for different heterotrimeric G-proteins or for other G-proteins, such as Rho GTPases. Versatility was further validated by implementing the biosensors in multiple contexts, from characterizing cancer-associated G-protein mutants to neurotransmitter signaling in primary neurons. Overall, the versatile biosensor design introduced here enables studying the activity of endogenous G-proteins in live cells with high fidelity, temporal resolution, and convenience.

DOI: 10.1016/j.cell.2020.06.020

Source: https://www.cell.com/cell/fulltext/S0092-8674(20)30752-2