小柯机器人

雄性小鼠受精需要基因座pi6
2020-06-30 21:03

美国麻萨诸塞大学医学院Phillip D. Zamore和Zhiping Weng研究组合作取得新进展。他们揭示了进化上保守的piRNA产生基因座第6号染色体(pi6)是雄性小鼠生育所必需的。这一研究成果发表在2020年6月29日的《自然-遗传学》上。

他们报告说,缺少来自pi6上保守的小鼠粗线piRNA基因座piRNA的雄性产生的精子,在获能和卵子受精方面存在缺陷。此外,由pi6-/-雄性亲本产生的杂合子胚胎在子宫内的活力降低。分子分析表明,pi6 piRNAs通过切割精子功能所需的蛋白质编码信使RNA来抑制基因表达。pi6还参与了piRNA-piRNA前体相互作用网络,该网络启动了10号染色体上第二个piRNA基因座以及pi6本身的piRNA产生。他们的数据建立了厚金属酸piRNA在精子发生和胚胎生存能力中的直接作用。

据介绍,在成年小鼠睾丸中包含> 80%的小RNA的粗线PIWI相互作用RNA(piRNA),可以结合和调节诸如microRNA的靶RNA,切割诸如短干扰RNA的靶点或完全缺乏生物学功能。尽管piRNA途径蛋白突变体是雄性不育的,但尚未发现任何哺乳动物piRNA产生基因座的生物学功能。

附:英文原文

Title: The evolutionarily conserved piRNA-producing locus pi6 is required for male mouse fertility

Author: Pei-Hsuan Wu, Yu Fu, Katharine Cecchini, Deniz M. zata, Amena Arif, Tianxiong Yu, Cansu Colpan, Ildar Gainetdinov, Zhiping Weng, Phillip D. Zamore

Issue&Volume: 2020-06-29

Abstract: Pachytene PIWI-interacting RNAs (piRNAs), which comprise >80% of small RNAs in the adult mouse testis, have been proposed to bind and regulate target RNAs like microRNAs, cleave targets like short interfering RNAs or lack biological function altogether. Although piRNA pathway protein mutants are male sterile, no biological function has been identified for any mammalian piRNA-producing locus. Here, we report that males lacking piRNAs from a conserved mouse pachytene piRNA locus on chromosome 6 (pi6) produce sperm with defects in capacitation and egg fertilization. Moreover, heterozygous embryos sired by pi6/ fathers show reduced viability in utero. Molecular analyses suggest that pi6 piRNAs repress gene expression by cleaving messenger RNAs encoding proteins required for sperm function. pi6 also participates in a network of piRNA–piRNA precursor interactions that initiate piRNA production from a second piRNA locus on chromosome 10, as well as pi6 itself. Our data establish a direct role for pachytene piRNAs in spermiogenesis and embryo viability.

DOI: 10.1038/s41588-020-0657-7

Source: https://www.nature.com/articles/s41588-020-0657-7

Nature Genetics:《自然—遗传学》,创刊于1992年。隶属于施普林格·自然出版集团,最新IF:25.455
官方网址:https://www.nature.com/ng/
投稿链接:https://mts-ng.nature.com/cgi-bin/main.plex


本期文章:《自然—遗传学》:Online/在线发表

分享到:

0