小柯机器人

可操控植物基因表达的光遗传学工具问世
2020-06-30 21:18

德国杜赛尔多夫大学Matias D. Zurbriggen研究组开发了可用于植物基因表达操控的光遗传学工具。相关论文于2020年6月29日在线发表在《自然—方法学》杂志上。

据研究人员介绍,光遗传学是用光控制细胞过程的遗传方法。它提供了对生物信号和代谢过程的时空、定量以及可逆地控制,从而克服了化学诱导系统的局限性。但是,光遗传学在植物研究方面滞后,因为生长所需的环境光会导致系统激活。

研究人员通过开发植物可用的光开关元件(PULSE)解决了这个问题,PULSE是一种在环境光下可逆地控制植物中基因表达的光遗传学工具。PULSE将蓝光调节抑制子和红光感应开关组合在一起。基因表达仅在红光下被激活,而在白光下或在黑暗中保持无效。在定量数学模型的支持下,研究人员对原生质体中的PULSE进行了表征并实现了高诱导率,并将其与基于CRISPR–Cas9的技术相结合,从而靶向合成信号和发育途径。研究人员应用PULSE来控制植物叶片中的免疫反应并产生了拟南芥转基因植物。
 
PULSE在植物研究和生物技术领域开辟了广泛的实验途径。
 
附:英文原文

Title: Optogenetic control of gene expression in plants in the presence of ambient white light

Author: Rocio Ochoa-Fernandez, Nikolaj B. Abel, Franz-Georg Wieland, Jenia Schlegel, Leonie-Alexa Koch, J. Benjamin Miller, Raphael Engesser, Giovanni Giuriani, Simon M. Brandl, Jens Timmer, Wilfried Weber, Thomas Ott, Rdiger Simon, Matias D. Zurbriggen

Issue&Volume: 2020-06-29

Abstract: Optogenetics is the genetic approach for controlling cellular processes with light. It provides spatiotemporal, quantitative and reversible control over biological signaling and metabolic processes, overcoming limitations of chemically inducible systems. However, optogenetics lags in plant research because ambient light required for growth leads to undesired system activation. We solved this issue by developing plant usable light-switch elements (PULSE), an optogenetic tool for reversibly controlling gene expression in plants under ambient light. PULSE combines a blue-light-regulated repressor with a red-light-inducible switch. Gene expression is only activated under red light and remains inactive under white light or in darkness. Supported by a quantitative mathematical model, we characterized PULSE in protoplasts and achieved high induction rates, and we combined it with CRISPR–Cas9-based technologies to target synthetic signaling and developmental pathways. We applied PULSE to control immune responses in plant leaves and generated Arabidopsis transgenic plants. PULSE opens broad experimental avenues in plant research and biotechnology.

DOI: 10.1038/s41592-020-0868-y

Source: https://www.nature.com/articles/s41592-020-0868-y

 

Nature Methods:《自然—方法学》,创刊于2004年。隶属于施普林格·自然出版集团,最新IF:28.467
官方网址:https://www.nature.com/nmeth/
投稿链接:https://mts-nmeth.nature.com/cgi-bin/main.plex


本期文章:《自然—方法学》:Online/在线发表

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