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研究揭示全基因组范围的重复基因保留
2020-06-28 14:39

加拿大多伦多大学Charles Boone、Brenda J. Andrews、美国明尼苏达大学Chad L. Myers等研究人员合作利用复杂的遗传相互作用分析,揭示了全基因组范围重复基因的保留。2020年6月26日,《科学》发表了这一成果。

研究人员报道了一个包含来自全基因组复制事件的酵母旁系同源物的系统型复杂遗传相互作用分析。每个旁系同源物缺失突变体的双基因相互作用映射,以及双突变体的三基因相互作用映射结果对其作用以及对其功能冗余的定量检测提供了新的见解。三基因相互作用分析可区分两类旁系同源物:功能上分歧较大的类群以及另一种保留更多功能重叠的类群。基因特征分析和建模表明,重复基因的进化轨迹是由功能和结构的纠缠因素共同决定的。
 
据了解,全基因组复制在包括人类基因组在内的许多生物的基因组进化中发挥了核心作用。大多数重复的基因被消除,影响长期重复保留的因素仍然知之甚少。 
 
附:英文原文

Title: Exploring whole-genome duplicate gene retention with complex genetic interaction analysis

Author: Elena Kuzmin, Benjamin VanderSluis, Alex N. Nguyen Ba, Wen Wang, Elizabeth N. Koch, Matej Usaj, Anton Khmelinskii, Mojca Mattiazzi Usaj, Jolanda van Leeuwen, Oren Kraus, Amy Tresenrider, Michael Pryszlak, Ming-Che Hu, Brenda Varriano, Michael Costanzo, Michael Knop, Alan Moses, Chad L. Myers, Brenda J. Andrews, Charles Boone

Issue&Volume: 2020/06/26

Abstract: Whole-genome duplication has played a central role in the genome evolution of many organisms, including the human genome. Most duplicated genes are eliminated, and factors that influence the retention of persisting duplicates remain poorly understood. We describe a systematic complex genetic interaction analysis with yeast paralogs derived from the whole-genome duplication event. Mapping of digenic interactions for a deletion mutant of each paralog, and of trigenic interactions for the double mutant, provides insight into their roles and a quantitative measure of their functional redundancy. Trigenic interaction analysis distinguishes two classes of paralogs: a more functionally divergent subset and another that retained more functional overlap. Gene feature analysis and modeling suggest that evolutionary trajectories of duplicated genes are dictated by combined functional and structural entanglement factors.

DOI: 10.1126/science.aaz5667

Source: https://science.sciencemag.org/content/368/6498/eaaz5667

Science:《科学》,创刊于1880年。隶属于美国科学促进会,最新IF:41.037
官方网址:https://www.sciencemag.org/
投稿链接:https://cts.sciencemag.org/scc/#/login

本期文章:《科学》:Volume 368 Issue 6498

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