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人类多能干细胞平台可用于建立新冠病毒感染模型
2020-06-23 14:00
来源：小柯机器人

美国康奈尔大学Shuibing Chen、Robert E. Schwartz、Todd Evans等研究人员合作开发出一个基于人类多能干细胞的平台，可用于建立新冠病毒（SARS-CoV-2）感染模型。该研究于2020年6月19日在线发表于《细胞—干细胞》。

研究人员开发了一个实验平台，该平台由人类多能干细胞（hPSC）衍生的细胞和类器官组成。突刺蛋白引起的病毒感染胰腺内分泌细胞、肝类器官、心肌细胞和多巴胺能神经元。最近的临床研究表明COVID-19与糖尿病有很强的联系。研究人员发现人胰腺β细胞和肝类器官容易被SARS-CoV-2感染，并使用了成人原代人胰岛以及成人肝细胞和胆管细胞类器官进一步验证。SARS-CoV-2感染引起趋化因子的大量表达，这在人类COVID-19肺尸检样本中也可以看到。hPSC衍生的细胞/类器官为理解人类组织对SARS-CoV-2感染的细胞反应以及对COVID-19的疾病建模提供了有力模型。
 
据悉，SARS-CoV-2导致了COVID-19大流行。目前迫切需要使用人类疾病相关细胞来研究SARS-CoV-2感染的生理模型。COVID-19的病理生理学包括呼吸衰竭，但涉及其他器官系统，包括肠、肝、心脏和胰腺。
 
附：英文原文

Title: A Human Pluripotent Stem Cell-based Platform to Study SARS-CoV-2 Tropism and Model Virus Infection in Human Cells and Organoids

Author: Liuliu Yang, Yuling Han, Benjamin E. Nilsson-Payant, Vikas Gupta, Pengfei Wang, Xiaohua Duan, Xuming Tang, Jiajun Zhu, Zeping Zhao, Fabrice Jaffré, Tuo Zhang, Tae Wan Kim, Oliver Harschnitz, David Redmond, Sean Houghton, Chengyang Liu, Ali Naji, Gabriele Ciceri, Sudha Guttikonda, Yaron Bram, Duc-Huy T. Nguyen, Michele Cioffi, Vasuretha Chandar, Daisy A. Hoagland, Yaoxing Huang, Jenny Xiang, Hui Wang, David Lyden, Alain Borczuk, Huanhuan Joyce Chen, Lorenz Studer, Fong Cheng Pan, David D. Ho, Benjamin R. tenOever, Todd Evans, Robert E. Schwartz, Shuibing Chen

Issue&Volume: 2020-06-19

Abstract: SARS-CoV-2 has caused the COVID-19 pandemic. There is an urgent need for physiological models to study SARS-CoV-2 infection using human disease-relevant cells. COVID-19 pathophysiology includes respiratory failure but involves other organ systems including gut, liver, heart, and pancreas. We present an experimental platform comprised of cell and organoid derivatives from human pluripotent stem cells (hPSCs). A Spike-enabled pseudo-entry virus infects pancreatic endocrine cells, liver organoids, cardiomyocytes, and dopaminergic neurons. Recent clinical studies show a strong association with COVID-19 and diabetes. We find that human pancreatic beta cells and liver organoids are highly permissive to SARS-CoV-2 infection, further validated using adult primary human islets and adult hepatocyte and cholangiocyte organoids. SARS-CoV-2 infection caused striking expression of chemokines, as also seen in primary human COVID-19 pulmonary autopsy samples. hPSC-derived cells/organoids provide valuable models for understanding the cellular responses of human tissues to SARS-CoV-2 infection and for disease modeling of COVID-19.

DOI: 10.1016/j.stem.2020.06.015

Source: https://www.cell.com/cell-stem-cell/fulltext/S1934-5909(20)30282-4

Cell Stem Cell：《细胞—干细胞》，创刊于2007年。隶属于细胞出版社，最新IF：25.269
官方网址：https://www.cell.com/cell-stem-cell/home
投稿链接：https://www.editorialmanager.com/cell-stem-cell/default.aspx


本期文章：《细胞—干细胞》：Online/在线发表

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