中国人民解放军总医院第五医学中心刘兵、新加坡免疫协作组Florent Ginhoux、暨南大学基础医学院兰雨等研究人员合作绘制了人类巨噬细胞的发育图谱。2020年5月20日,《自然》在线发表了这一成果。
Title: Deciphering human macrophage development at single-cell resolution
Author: Zhilei Bian, Yandong Gong, Tao Huang, Christopher Z. W. Lee, Lihong Bian, Zhijie Bai, Hui Shi, Yang Zeng, Chen Liu, Jian He, Jie Zhou, Xianlong Li, Zongcheng Li, Yanli Ni, Chunyu Ma, Lei Cui, Rui Zhang, Jerry K. Y. Chan, Lai Guan Ng, Yu Lan, Florent Ginhoux, Bing Liu
Issue&Volume: 2020-05-20
Abstract: Macrophages are the first cells of the nascent immune system to emerge during embryonic development. In mice, embryonic macrophages infiltrate developing organs, where they differentiate symbiotically into tissue-resident macrophages (TRMs)1. However, our understanding of the origins and specialization of macrophages in human embryos is limited. Here we isolated CD45+ haematopoietic cells from human embryos at Carnegie stages 11 to 23 and subjected them to transcriptomic profiling by single-cell RNA sequencing, followed by functional characterization of a population of CD45+CD34+CD44+ yolk sac-derived myeloid-biased progenitors (YSMPs) by single-cell culture. We also mapped macrophage heterogeneity across multiple anatomical sites and identified diverse subsets, including various types of embryonic TRM (in the head, liver, lung and skin). We further traced the specification trajectories of TRMs from either yolk sac-derived primitive macrophages or YSMP-derived embryonic liver monocytes using both transcriptomic and developmental staging information, with a focus on microglia. Finally, we evaluated the molecular similarities between embryonic TRMs and their adult counterparts. Our data represent a comprehensive characterization of the spatiotemporal dynamics of early macrophage development during human embryogenesis, providing a reference for future studies of the development and function of human TRMs.
DOI: 10.1038/s41586-020-2316-7
Source: https://www.nature.com/articles/s41586-020-2316-7
Nature:《自然》,创刊于1869年。隶属于施普林格·自然出版集团,最新IF:69.504
官方网址:http://www.nature.com/
投稿链接:http://www.nature.com/authors/submit_manuscript.html
本期文章:《自然》:Online/在线发表