美国加州大学洛杉矶分校April D. Pyle研究组合作取得最新进展。他们发现人类骨骼肌图谱可确定整个发育过程中和人类多能干细胞（hPSC）中的干细胞和祖细胞的轨迹。2020年5月11日的《细胞-干细胞》杂志发表了这一研究成果。
类骨骼肌发生的发育轨迹以及祖细胞和干细胞状态之间的转换尚不清楚。研究人员使用单细胞RNA测序来分析胚胎、胎儿和出生后阶段的人体骨骼肌组织。经由计算机模拟，他们确定了肌源性以及其他细胞类型，并构建了整个发育过程中人类骨骼肌个体发育的“路线图”。
以类似的方式，他们还概述了从多个hPSC肌源性分化方案产生的异质细胞培养，并将hPSC衍生的肌源祖细胞定位到胚胎到胎儿的过渡期。他们发现差异丰富的生物过程，并发现了在不同的成肌阶段存在的共同调控的基因网络和转录因子。
这项工作为增进人类肌发生的过程提供了资源。它还提供了一个工具，可以更好地了解hPSC的成肌祖细胞，用于基于骨骼肌的再生医学中的翻译应用。
附：英文原文
Title: A Human Skeletal Muscle Atlas Identifies the Trajectories of Stem and Progenitor Cells across Development and from Human Pluripotent Stem Cells
Author: Haibin Xi, Justin Langerman, Shan Sabri, Peggie Chien, Courtney S. Young, Shahab Younesi, Michael Hicks, Karen Gonzalez, Wakana Fujiwara, Julia Marzi, Simone Liebscher, Melissa Spencer, Ben Van Handel, Denis Evseenko, Katja Schenke-Layland, Kathrin Plath, April D. Pyle
Issue&Volume: 2020-05-11
Abstract: The developmental trajectory of human skeletal myogenesis and the transition betweenprogenitor and stem cell states are unclear. We used single-cell RNA sequencing toprofile human skeletal muscle tissues from embryonic, fetal, and postnatal stages.In silico, we identified myogenic as well as other cell types and constructed a “roadmap” ofhuman skeletal muscle ontogeny across development. In a similar fashion, we also profiledthe heterogeneous cell cultures generated from multiple human pluripotent stem cell(hPSC) myogenic differentiation protocols and mapped hPSC-derived myogenic progenitorsto an embryonic-to-fetal transition period. We found differentially enriched biologicalprocesses and discovered co-regulated gene networks and transcription factors presentat distinct myogenic stages. This work serves as a resource for advancing our knowledgeof human myogenesis. It also provides a tool for a better understanding of hPSC-derivedmyogenic progenitors for translational applications in skeletal muscle-based regenerativemedicine.
DOI: 10.1016/j.stem.2020.04.017
Source: https://www.cell.com/cell-stem-cell/fulltext/S1934-5909(20)30156-9
Cell Stem Cell：《细胞—干细胞》，创刊于2007年。隶属于细胞出版社，最新IF：25.269
官方网址：https://www.cell.com/cell-stem-cell/home
投稿链接：https://www.editorialmanager.com/cell-stem-cell/default.aspx
本期文章：《细胞—干细胞》：Online/在线发表
