小柯机器人

美国芝加哥大学Marcus R. Clark及其研究团队探究了生发中心（GC）内新型特化细胞的状态和空间分布。该项研究成果2020年4月27日在线发表于《自然—免疫学》。

研究人员发现，在明区（LZ）中进行选择后，B细胞迁移到典型暗区（DZ）中的特定位点，该DZ包含可识别的体巨噬细胞，并且这群细胞处在细胞分裂的位点。随后，增殖的DZ（DZp）细胞过渡到较大的DZ中，成为分化DZ（DZd）细胞，然后重新进入LZ。多维分析揭示了每个亚群中不同的分子基序，这与观察到的不兼容功能区室化相称。

这些数据提供了一个新的三细胞亚群模型，该模型既揭示了重要的GC功能，又探明了体液适应性免疫的分子基序。

据了解，在GC中，复杂且高度协调的分子基序是平衡增殖、体细胞超突变和选择所必须的，其提供了有效的体液免疫并防止基因组不稳定和肿瘤转化。与这种复杂性相反，GC B细胞主要分为两个主要亚群，DZ和LZ细胞。

附：英文原文

Title: Novel specialized cell state and spatial compartments within the germinal center

Author: Domenick E. Kennedy, Michael K. Okoreeh, Mark Maienschein-Cline, Junting Ai, Margaret Veselits, Kaitlin C. McLean, Yogesh Dhungana, Hong Wang, Junmin Peng, Hongbo Chi, Malay Mandal, Marcus R. Clark

Issue&Volume: 2020-04-27

Abstract: Within germinal centers (GCs), complex and highly orchestrated molecular programs must balance proliferation, somatic hypermutation and selection to both provide effective humoral immunity and to protect against genomic instability and neoplastic transformation. In contrast to this complexity, GC B cells are canonically divided into two principal populations, dark zone (DZ) and light zone (LZ) cells. We now demonstrate that, following selection in the LZ, B cells migrated to specialized sites within the canonical DZ that contained tingible body macrophages and were sites of ongoing cell division. Proliferating DZ (DZp) cells then transited into the larger DZ to become differentiating DZ (DZd) cells before re-entering the LZ. Multidimensional analysis revealed distinct molecular programs in each population commensurate with observed compartmentalization of noncompatible functions. These data provide a new three-cell population model that both orders critical GC functions and reveals essential molecular programs of humoral adaptive immunity.

DOI: 10.1038/s41590-020-0660-2

Source: https://www.nature.com/articles/s41590-020-0660-2

Nature Immunology：《自然—免疫学》，创刊于2000年。隶属于施普林格·自然出版集团，最新IF：31.25
官方网址：https://www.nature.com/ni/
投稿链接：https://mts-ni.nature.com/cgi-bin/main.plex