小柯机器人

近日，德国杜塞尔多夫大学Wolf B. Frommer和美国密苏里大学Bing Yang等研究人员合作研发了水稻抗枯萎病诊断试剂盒。2019年10月28日，国际知名学术期刊《自然—生物技术》在线发表了这一成果。

研究人员报道了一种诊断试剂盒，可用于在野外分析细菌疫病并鉴定合适的抗性系。具体来说，这个试剂盒包括SWEET启动子数据库、用于检测SWEET诱导的RT–PCR引物、工程化的报告稻系从而可视化SWEET蛋白的积累以及敲除水稻系以鉴定细菌分离株中的毒力机制。研究人员还开发了CRISPR – Cas9基因组编辑的Kitaake水稻，以评估EBE突变抗性的功效，以及可预测特定地理区域最佳抗性基因集的软件，并开发了两个具有抗性的“超级”水稻系，这些水稻系将使农民能够种植最有可能抵抗稻疫病的植物。

据介绍，抗枯萎的水稻系是细菌枯萎病（由Xanthomonas oryzae pv. Oryzae引起）最有效的解决方案。关键抗性机制涉及SWEET基因作为易感性因子。细菌转录激活因子样（TAL）效应子与SWEET基因启动子中的效应子结合元件（EBE）结合并诱导SWEET基因。TAL效应子无法识别的EBE变体阻止诱导，从而引起抗性。

附：英文原文

Title: Diagnostic kit for rice blight resistance

Author: Joon-Seob Eom, Dangping Luo, Genelou Atienza-Grande, Jungil Yang, Chonghui Ji, Van Thi Luu, Jos C. Huguet-Tapia, Si Nian Char, Bo Liu, Hanna Nguyen, Sarah Maria Schmidt, Boris Szurek, Casiana Vera Cruz, Frank F. White, Ricardo Oliva, Bing Yang, Wolf B. Frommer

Issue&Volume: 2019-10-28

Abstract: Blight-resistant rice lines are the most effective solution for bacterial blight, caused by Xanthomonas oryzae pv. oryzae (Xoo). Key resistance mechanisms involve SWEET genes as susceptibility factors. Bacterial transcription activator-like (TAL) effectors bind to effector-binding elements (EBEs) in SWEET gene promoters and induce SWEET genes. EBE variants that cannot be recognized by TAL effectors abrogate induction, causing resistance. Here we describe a diagnostic kit to enable analysis of bacterial blight in the field and identification of suitable resistant lines. Specifically, we include a SWEET promoter database, RT–PCR primers for detecting SWEET induction, engineered reporter rice lines to visualize SWEET protein accumulation and knock-out rice lines to identify virulence mechanisms in bacterial isolates. We also developed CRISPR–Cas9 genome-edited Kitaake rice to evaluate the efficacy of EBE mutations in resistance, software to predict the optimal resistance gene set for a specific geographic region, and two resistant ‘mega’ rice lines that will empower farmers to plant lines that are most likely to resist rice blight.

DOI: 10.1038/s41587-019-0268-y

Source: https://www.nature.com/articles/s41587-019-0268-y

Nature Biotechnology：《自然—生物技术》，创刊于1996年。隶属于施普林格·自然出版集团，最新IF：68.164
官方网址：https://www.nature.com/nbt/
投稿链接：https://mts-nbt.nature.com/cgi-bin/main.plex