博文
David Wang实验室建立了第一个天然的病毒感染线虫模型
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病毒感染细胞的模型，诞生了无数大牛，量产着无数科研成果和科研人员，现在病毒感染线虫的模型终于建立了，在线虫这个无数基因首先被命名被定义的归零地，开展病毒宿主互作的研究潜力是极大的，如果能买股票的话，我立马入手，行情看涨啊~
原始文献：
http://www.ncbi.nlm.nih.gov/pubmed/25078701
P.S.正事儿说完了，发几句牢骚：
为什么只有那个不待见的搜索引擎能找到我想找的David Wang
都是搜索出来的第一条链接：
首先是Bing
然后是百度
最后是某个不待见的搜索引擎
- David Wang, PhD - Department of Pathology & Immunologywww.pathology.wustl.edu/faculty/index.php?user...翻译此页Research. Research in the Wang laboratory is situated at the interface of molecular and cellular virology, genomics and bioinformatics with the overall goal of ...
这才是我要找的那个David Wang，不是纽约的fashion photographer，也不是Senior Vice President王啟尚，而是华盛顿大学的教授David Wang。我关注他是因为他在感染线虫的病毒模型的一系列尝试，今天的这篇J Virol算是一个宣告，第一个感染线虫的病毒模型终于建立了！引用“To date, Orsay virus is the first and the only identified virus capable of naturally infecting Caenorhabditis elegans.”
http://www.ncbi.nlm.nih.gov/pubmed/25078701
注意到以前关于线虫 病毒 感染模型的报道
http://www.bbioo.com/experiment/24-87219-1.html
References:
{1} Lu et al. Nature 2005, 436:1040-3 [PMID:16107851].
{2} Wilkins et al. Nature 2005, 436:1044-7 [PMID:16107852].
{3} Schott et al. Proc Natl Acad Sci U S A 2005, 102:18420-4 [PMID:16339901].
以线虫作为病毒感染模型的想法并不新，但这些不是天然感染线虫的病毒。比如我所关心的天然病毒感染 表面细胞 比如 肠道 后，最终会 到哪里去 （比如会不会 最终定居于 神经细胞等 寿命 很长的细胞里），这样的问题，似乎能 在透明的线虫里看到答案。
2014/8/21
病毒晶体结构都出来了~
1Department of BioSciences, Rice University, Houston, TX 77005;
2Graduate Program in Structural and Computational Biology and Molecular Biophysics andVerna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030; and.
3Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030; and.
4Departments of Molecular Microbiology andPathology and Immunology, Washington University School of Medicine in St. Louis, St. Louis, MO 63110.
5Department of BioSciences, Rice University, Houston, TX 77005; ytao@rice.edu.
Orsay, the first virus discovered to naturally infect Caenorhabditis elegans or any nematode, has a bipartite, positive-sense RNA genome. Sequence analyses show that Orsay is related to nodaviruses, but molecular characterizations of Orsay reveal several unique features, such as the expression of a capsid-δ fusion protein and the use of an ATG-independent mechanism for translation initiation. Here we report the crystal structure of an Orsay virus-like particle assembled from recombinant capsid protein (CP). Orsay capsid has a T = 3 icosahedral symmetry with 60 trimeric surface spikes. Each CP can be divided into three regions: an N-terminal arm that forms an extended protein interaction network at the capsid interior, an S domain with a jelly-roll, β-barrel fold forming the continuous capsid, and a P domain that forms surface spike projections. The structure of the Orsay S domain is best aligned to T = 3 plant RNA viruses but exhibits substantial differences compared with the insect-infecting alphanodaviruses, which also lack the P domain in their CPs. The Orsay P domain is remotely related to the P1 domain in calicivirus and hepatitis E virus, suggesting a possible evolutionary relationship. Removing the N-terminal arm produced a slightly expanded capsid with fewer nucleic acids packaged, suggesting that the arm is important for capsid stability and genome packaging. Because C. elegans-Orsay serves as a highly tractable model for studying viral pathogenesis, our results should provide a valuable structural framework for further studies of Orsay replication and infection.
crystallography; microscopy; virology
https://wap.sciencenet.cn/blog-89798-816489.html
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