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Protoplast culture of rice
孟祥兵 2008-7-14 18:36
Protoplast culture of rice ( Oryza sativa L.) using media solidified with agarose J. A. Thompson, R. Abdullah * and E. C. Cocking Plant Genetic Manipulation Group, Department of Botany, University of Nottingham, University Park, Nottingham NG7 2RD, U.K. Received 17 February 1986;&n ...
个人分类: 生活点滴|5742 次阅读|没有评论
今天我的博客浏览次数为8888
孟祥兵 2008-7-8 18:39
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水稻转化要素之三:种子消毒
孟祥兵 2008-6-26 19:35
Surface Sterilization of Plant Explants and Orchid Seed To avoid bacterial and fungal growth, which is detrimental to culture growth, explants are surface-sterilized before they are used to establish in vitro axenic cultures. The most common disinfectants are listed below with the concentrat ...
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水稻转化要素之二:培养基灭菌
孟祥兵 2008-6-26 19:31
Plant tissue culture media are generally sterilized by autoclaving at 121 C and 1.05 kg/cm2 (15-20 psi). The time required for sterilization depends upon the volume of medium in the vessel. The minimum times required for sterilization of different volumes of medium are listed below. It is advis ...
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水稻转化要素之一:Phytagel
孟祥兵 2008-6-26 19:18
Phytagel is an agar substitute produced from a bacterial substrate composed of glucuronic acid葡萄糖醛酸, rhamnose 鼠李糖 and glucose. It produces a clear, colorless, high-strength gel, which aids in detection of microbial contamination. Phytagel provides an economical alternative to agar as a gell ...
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BIOFUELS
孟祥兵 2008-3-19 08:07
BIO
个人分类: 生活点滴|3201 次阅读|没有评论
玉米基因组数据列表
孟祥兵 2008-3-19 08:03
MAGI 玉米基因组数据列表 MAGI 4.0 Contigs and Singletons 727,781 Sequences 201.0 MB P ...
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Plant barcodes
孟祥兵 2008-3-19 08:02
Plant barcodes 18S rDNA (1855 bp), rbcL (1428 bp), and atpB (1450 bp) representing a total of 4733 bp Plastid rbcL: the large subunit of Rubisco (ribulose bisphosphate carboxylase) 1,5- 二磷酸核酮糖羧化酶 / 加氧酶大亚基 ribulose-1,5-bisp ...
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水稻组织培养-分化生根的激素
孟祥兵 2008-3-19 07:57
生长素 auxin 植物内源生长素:吲哚乙酸( IAA ),吲哚丁酸( IBA ),苯乙酸( PAA ) 结构特点:有一个芳香环和羧基侧链,二者之间有一个芳香环或氧原子间隔。 人工合成生长素: 2 , 4- 二氯苯氧乙酸( 2 , 4- D )和 - 萘乙酸 NAA ...
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水稻组织培养-悬浮细胞培养
孟祥兵 2008-2-29 08:11
悬浮细胞培养文献
个人分类: 生活点滴|4000 次阅读|没有评论

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