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Plant Cell:草莓NAC转录因子FaRIF控制果实成熟

已有 616 次阅读 2021-3-1 10:52 |个人分类:每日摘要|系统分类:论文交流

The NAC transcription factor FaRIF controls fruit ripening in strawberry

第一作者Carmen Martín-Pizarro

第一单位西班牙马拉加大学

通讯作者David Posé


 Abstract 


背景回顾+问题In contrast to climacteric fruits such as tomato, the knowledge on key regulatory genes controlling the ripening of strawberry, a non-climacteric fruit, is still limited. NAC transcription factors mediate different developmental processes in plants. 


主要发现:Here, we identified and characterized FaRIF (Ripening Inducing Factor), a NAC transcription factor that is highly expressed and induced in strawberry receptacles during ripening


结果1-功能验证-沉默、异位表达和过表达:Functional analyses based on stable transgenic lines aimed at silencing FaRIF by RNA interference, either from a constitutive promoter or the ripe receptacle-specific EXP2 promoter, as well as overexpression lines showed that FaRIF controls critical ripening-related processes such as fruit softening and pigment and sugar accumulation. 


结果2-生理、转录+代谢组学:Physiological, metabolome and transcriptome analyses of receptacles of FaRIF-silenced and overexpression lines point to FaRIF as a key regulator of strawberry fruit ripening from early developmental stages, controllingabscisic acid (ABA) biosynthesis and signaling, cell wall degradation and modification, the phenylpropanoid pathway, volatiles production, and the balance of the aerobic/anaerobic metabolism. 


结论:FaRIF is therefore a target to be modified/edited to control the quality of strawberry fruits.

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 摘 要 


与番茄等具有跃变型果实不同,目前我们对于控制草莓等非跃变型果实成熟的关键调控基因仍然所知甚少。NAC转录因子介导了植物发育的多个进程。本文中,作者鉴定了一个草莓的果实成熟诱导因子FaRIF,属于NAC转录因子,在草莓果成熟过程中,在花托中被诱导高表达。作者基于稳定遗传转化对FaRIF基因进行了功能验证,包括RNA干扰技术介导的FaRIF基因沉默、组成型启动子或成熟花托特异性EXP2启动子诱导表达、以及过表达试验等,发现FaRIF能够控制与成熟相关的重要进程,比如果实变软、着色和糖积累。对于FaRIF沉默和过表达株系的花托进行生理、代谢和转录组分析,结果显示是FaRIF在草莓果实成熟的早期发育阶段就开始发挥关键调控作用,控制脱落酸的生物合成和信号转导、细胞壁降解和修饰、苯丙烷途径、挥发性化合物产生以及有氧/无氧代谢的平衡。因此,FaRIF是一个控制草莓果实质量的潜在修饰和编辑对象。


doi: https://doi.org/10.1093/plcell/koab070


Journal: Plant Cell

Published date: Feb 24, 2021



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