A network of transcriptional repressors modulates auxin responses

第一作者：Jekaterina Truskina

第一单位：法国里昂大学

通讯作者：Teva Vernoux

 Abstract 

大背景：The regulation of signalling capacity, combined with the spatiotemporal distribution of developmental signals themselves, is pivotal in setting developmental responses in both plants and animals.

小背景：The hormone auxin is a key signal for plant growth and development that acts through the AUXIN RESPONSE FACTOR (ARF) transcription factors. A subset of these, the conserved class A ARFs, are transcriptional activators of auxin-responsive target genes that are essential for regulating auxin signalling throughout the plant lifecycle.

提出问题：Although class A ARFs have tissue-specific expression patterns, how their expression is regulated is unknown.

结果1-染色质修饰与可及性：Here we show, by investigating chromatin modifications and accessibility, that loci encoding these proteins are constitutively open for transcription.

结果2-转录调控因子鉴定：Through yeast one-hybrid screening, we identify the transcriptional regulators of the genes encoding class A ARFs from Arabidopsis thaliana and demonstrate that each gene is controlled by specific sets of transcriptional regulators.

结果3-转录抑制作用：Transient transformation assays and expression analyses in mutants reveal that, in planta, the majority of these regulators repress the transcription of genes encoding class A ARFs.

结论：These observations support a scenario in which the default configuration of open chromatin enables a network of transcriptional repressors to regulate expression levels of class A ARF proteins and modulate auxin signalling output throughout development.

 通讯作者 

doi: 10.1038/s41586-020-2940-2

Journal: Nature

Published online: Nov 18, 2020