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韩春雨Nature Biotechnology论文受到国际学界高度关注

已有 10842 次阅读 2016-5-8 16:26 |个人分类:科研动态|系统分类:观点评述

致敬韩春雨老师-“不鸣则已,一鸣惊人”在Nature Biotech发表重 精选

http://blog.sciencenet.cn/blog-300826-975762.html

5月2日论文发表后受到国际学界的关注,我们会继续跟踪国际反响和评论。

Altmetric替代计量得分  55分  https://www.altmetric.com/details/7114638

Mentioned byTwitter81 tweeters       微博关注81条
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Unknown3948%
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Science communicators (journalists, bloggers, editors)  科学媒体人11%



韩春雨老师近照(来源:河北科技大学微信号)


2016 May 2. doi: 10.1038/nbt.3547. [Epub ahead of print]

DNA-guided genome editing using the Natronobacterium gregoryi Argonaute.

Author information
  • 1Department of Bioscience and Bioengineering, Hebei University of Science and Technology, Shijiazhuang, Hebei, China.

  • 2Department of Physiology, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.

Abstract

The RNA-guided endonuclease Cas9 has made genome editing a widely accessible technique. Similar to Cas9, endonucleases from the Argonaute protein family also use oligonucleotides as guides to degrade invasive genomes. Here we report that the Natronobacterium gregoryi Argonaute (NgAgo) is a DNA-guided endonuclease suitable for genome editing in human cells. NgAgo binds 5' phosphorylated single-stranded guide DNA (gDNA) of ~24 nucleotides, efficiently creates site-specific DNA double-strand breaks when loaded with the gDNA. The NgAgo-gDNA system does not require a protospacer-adjacent motif (PAM), as does Cas9, and preliminary characterization suggests a low tolerance to guide-target mismatches and high efficiency in editing (G+C)-rich genomic targets.

PMID: 27136078 [PubMed - as supplied by publisher]

Gao F . Shen XZ . Jiang F . Wu Y . Han C .
Department of Bioscience and Bioengineering, Hebei University of Science and Technology, Shijiazhuang, Hebei, China.
来源: Nat Biotechnol ( P 1087-0156 E 1546-1696 ) IF:41.514 H指数:251 年: 2016
PMID: 27136078 [Pubmed] 生物工程与应用微生物,1区

  • 文摘

  • Link@SJ-HOSPITAL

  • 相关文献

  • Altmetric得分:

  • About this score
    • In the top 5% of all research outputs scored by Altmetric
    • High score compared to outputs of the same age (96th percentile)
    • Good score compared to outputs of the same age and source (65th percentile)
    Mentioned byTwitter81 tweeters
    Readers onMendeley35 Mendeley
    Title
    DNA-guided genome editing using the Natronobacterium gregoryi Argonaute
    Published in
    Nature Biotechnology, May 2016
    DOI10.1038/nbt.3547
    Pubmed ID
    Authors

    Feng Gao, Xiao Z Shen, Feng Jiang, Yongqiang Wu, Chunyu Han, Gao, Feng, Shen, Xiao Z, Jiang, Feng, Wu, Yongqiang, Han, Chunyu

    Abstract

    The RNA-guided endonuclease Cas9 has made genome editing a widely accessible technique. Similar to Cas9, endonucleases from the Argonaute protein family also use oligonucleotides as guides to degrade invasive genomes. Here we report that the Natronobacterium gregoryi Argonaute (NgAgo) is a DNA-guided endonuclease suitable for genome editing in human cells. NgAgo binds 5' phosphorylated single-stranded guide DNA (gDNA) of ~24 nucleotides, efficiently creates site-specific DNA double-strand breaks when loaded with the gDNA. The NgAgo-gDNA system does not require a protospacer-adjacent motif (PAM), as does Cas9, and preliminary characterization suggests a low tolerance to guide-target mismatches and high efficiency in editing (G+C)-rich genomic targets.

    Twitter Demographics
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    Mendeley readers
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