alittleone的个人博客

程琳
访问数:118690
教育情况:中南大学,博士
研究领域:医学科学->药理学->临床药理
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Acetic acid (醋酸或乙酸) 溶液的配制

问:如何配制1M的acetic acid溶液? 答:先计算母液的浓度。0.99 (purity)* 1.049 g/mL (density) / 60.05 g/ m ol ( molecular weight ) =0.0173 mol/mL = 13.7 mol/L = 17.3 M 所以,1M acetic acid溶液是将母液稀释17.3倍得到的,即1mL母液溶于16.3 mL dH2O中。
2024-2-18 12:31

LC-MS

Liquid chromatography-massspectrometry has been used to detect the 3 different change levels. 1. Drug concentration 2. Drug metabolites (metabolomics) 3. Proteinmics (the protein level changes due to drug intake) Sam runs the University of Chicago Core of ...
2023-12-1 05:34

WB control loading antibody

2023-5-25 21:40

Seahorse notes

Oligomycin: a complex V inhibitor, derive ATP-linked respiration and proton leak respiration. FCCP: ie. proton (H + ), for the maximum capacity ofH + Rotenone: shut down ETC (electron transport chain) function, revealing the non-mitochondrial respiration.non-mutdrome consumptio ...
2023-5-17 05:48

Immune cell markers

CD3: Universal T cells CD4: helper T cells CD8: cytotoxic T cells CD19: B cells CD21: mature B cells/ follicular dendritic cells CD25: T regulatory cells FoxP3: T regulatory cells (more specific) IFN: interferon cytokines
2023-5-5 03:33

Cell death markers

Bcl-2 superfamily Pro-apoptotic Bax, Bid, Bok, Bik, Bak, Noxa, Bim, Puma, Bad Anti-apoptotic BCL-2, A1, BCL-XL, BCL-W, BLFL-1, BCL-B, MCL-1 Caspases Initiator: Caspase 2,Caspase 8,Caspase 9,Caspase 10 Effector:Caspase 3,Caspase 6,Casp ...
2023-4-9 10:15

glutamine vs. glutamate

glutamine vs. glutamate (glutamate =L-Glutamic acid ) 谷氨酰胺vs.谷氨酸 它们是不同的氨基酸 Glutamine (谷氨酰胺)is a conditionally essential amino acid that has various functions of the body. Glutamate (谷氨酸) is a non-essential amino acid whic ...
2023-3-29 00:07

how to know if a gene is a transcription factor?

To qualify as a transcription factor, a protein must possess two qualities. 1) Ability to bind to DNA and 2) recruit RNA polymerase/alter transcription of a gene. The most common GTFs are TFIIA, TFIIB, TFIID (see also TATA binding protein), TFIIE, TFIIF, and TFIIH . ...
2023-3-25 11:01

PCR中DMSO该加多少浓度可以提高反应的特异性?

一般而言,加入终浓度2.5%的DMSO可增加PCR的特异性。KJ建议加终浓度为5%的DMSO,可降解二聚体结构产生的可能。
2023-3-22 23:13
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