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你是猴子请来的啥?

已有 3550 次阅读 2014-2-27 18:11 |系统分类:科研笔记

这是过年的时候在家刷文献就看到的了(可见我在家有多无聊),今天瞎逛Cell看到居然还在首页就拿出来说说。


沙老师、季老师和黄老师联手的Cell主刊大作,引发了很多大牛杂志的评论~

早在去年年初那篇小鼠CRISPR的文章出来后就猜到CRISPR技术会非常之火,以至于我在实验室年终大组会上也介绍了相关文献。沙老师他们果然走在科研前沿,第一只CRISPR修饰猴子也真算是划时代。enjoy~

http://www.cell.com/abstract/S0092-8674(14)00079-8


Generation of Gene-Modified Cynomolgus Monkey via Cas9/RNA-Mediated Gene Targeting in One-Cell Embryos

  • Highlights

  • CRISPR/Cas9-mediated simultaneous targeting of multiple genes in monkey embryos

  • Demonstrates an efficient and reliable approach for genome modified monkey generation

  • Achieves Ppar-g and Rag1 double mutation in monkeys in one step

Summary

Monkeys serve as important model species for studying human diseases and developing therapeutic strategies, yet the application of monkeys in biomedical researches has been significantly hindered by the difficulties in producing animals genetically modified at the desired target sites. Here, we first applied the CRISPR/Cas9 system, a versatile tool for editing the genes of different organisms, to target monkey genomes. By coinjection of Cas9 mRNA and sgRNAs into one-cell-stage embryos, we successfully achieve precise gene targeting in cynomolgus monkeys. We also show that this system enables simultaneous disruption of two target genes (Ppar-γ and Rag1) in one step, and no off-target mutagenesis was detected by comprehensive analysis. Thus, coinjection of one-cell-stage embryos with Cas9 mRNA and sgRNAs is an efficient and reliable approach for gene-modified cynomolgus monkey generation.


 





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