香蕉枯萎病是一种毁灭病害,是国际植物检疫对象,由尖孢镰刀菌古巴专化型侵染引起。在我省主要分布1号和4号小种,1号小种主要为害粉蕉,4号小种为害粉蕉和香蕉(巴西蕉,主要吃的品种)。病原菌在土壤中存活时间达数年之久,严重的田块甚至绝收。
由于4号对当前栽培品种威胁很大,所以大家都在想对其进行防控。 我们基因组揭示4号进化速率,包括转座子变异都要比1号大,这些变异可能是导致4号能感染巴西蕉。
本项目主要完成者有福建农林大写云英子博士, 宋爱霞同学, 王宗华教授 和张亮生教授。
在此工作完成基础之上,我们与王宗华教授课题组还继续展开合作,对4号小种感染巴西蕉机制做进一步分析,希望能解析其中感染机制。
Mol Plant Microbe Interact. 2019 May 7. doi: 10.1094/MPMI-03-19-0063-A. [Epub ahead of print]
Genome data of Fusarium oxysporum f. sp. cubense race 1 and tropical race 4 isolates using long-read sequencing.
Yun Y1,2, Song A3, Bao J4, Chen S5, Lu S6, Cheng C7, Zheng W8, Wang Z9,10, Zhang L11.
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Abstract
Fusarium wilt of banana is caused by the soil-borne fungal pathogen Fusarium oxysporum f. sp. cubense (Foc). We generated two chromosome-level assemblies of Foc race 1 and tropical race 4 strains using single-molecule real-time sequencing. The Foc1 and FocTR4 assemblies had 35 and 29 contigs with contig N50 lengths of 2.08 Mb and 4.28 Mb, respectively. These two new references genomes represent a greater than 100-fold improvement over the contig N50 statistics of the previous short read-based Foc assemblies. The two high-quality assemblies reported here will be a valuable resource for the comparative analysis of Foc races at the pathogenic levels.
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