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已有 3432 次阅读 2014-5-23 17:37 |个人分类:生活点滴|系统分类:论文交流

http://engineering.cae.cn/fase/EN/10.15302/J-FASE-2014010


Transcriptome resources and genome-wide marker development for Japanese larch (Larix kaempferi) 


LI Wanfeng, HAN Suying, QI Liwang, ZHANG Shougong

Abstract  

While the differential responses of trees to changes in climatic and environmental conditions have been demonstrated as they age, the underlying mechanisms and age control of tree growth and development are complex and poorly understood particularly at a molecular level. In this paper, we present a transcriptome analysis of Larix kaempferi, a deciduous conifer that is widely-grown in the northern hemisphere and of significant ecological and economic value. Using high-throughput RNA sequencing, we obtained about 26 million reads from the stems of 1-, 2-, 5-, 10-, 25- and 50-year-old L. kaempferi trees. Combining these with the published Roche 454 sequencing reads and the expressed sequence tags (both mainly from Larix embryogenic cell cultures), we assembled 26670549 reads into 146786 transcripts, of which we annotated 79182 to support investigations of the molecular basis of tree aging and adaption, somatic embryogenesis and wood formation. Using these sequences we also identified many single-nucleotide polymorphisms, simple sequence repeats, and insertion and deletion markers to assist breeding and genetic diversity studies of Larix.

Key wordsLarix    transcriptome    age    wood formation    somatic embryogenesis    molecular marker    

 



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