常用植物培养基母液

 

20х N6 major salts

KNO₃            56.6g/L

(NH₄)₂SO₄        9.26 g/L

KH₂PO₄          8.00 g/L

MgSO₄.7H₂O      3.7 g/L

CaCl₂.2H₂O       3.32 g/L

 

20хMS major salts

KNO₃        38g/L

NH₄NO₃      33 g/L

KH₂PO₄      3.4 g/L

MgSO₄.7H₂O  7.4 g/L

CaCl₂.2H₂O   8.8 g/L

 

20хCC major salts

KNO₃        24.24g/L

NH₄NO₃      12.8 g/L

KH₂PO₄      2.72 g/L

MgSO₄.7H₂O  4.94 g/L

CaCl₂.2H₂O   11.76 g/L

 

20хAA major salts

KCl              59 g/L

MgSO₄.7H₂O    10 g/L

CaCl₂.2H₂O     3 g/L

Na₂H₂PO₄       3 g/L

 

100хN6 minor salts

MnSO₄.4H₂O         440 mg/L   

H₃BO₃                      160 mg/L 

ZnSO₄.7H₂O          150 mg/L 

KI                   80 mg/L

 

100хB5 minor salts

MnSO₄.4H₂O             1320 mg/L

H₃BO₃                             300 mg/L

ZnSO₄.7H₂O              200 mg/L

Na₂MoO₄.2H₂O            25 mg/L   

CuSO₄.5H₂O               2.5 mg/L

CoCl₂.6H₂O                   2.5 mg/L

KI                           75 mg/L

 

100хMS minor salts

MnSO₄.4H₂O                        2230 mg/L

H₃BO₃                                             620 mg/L

ZnSO₄.7H₂O                        860mg/L

Na₂MoO₄.2H₂O                       25 mg/L

CuSO₄.5H₂O                         2.5 mg/L

CoCl₂.6H₂O                          2.5 mg/L

KI                                  83 mg/L

 

100хCC minor salts

MnSO₄.4H₂O                        1115 mg/L

H₃BO₃                                             310 mg/L

ZnSO₄.7H₂O                         576 mg/L

Na₂MoO₄.2H₂O                      24 mg/L

CuSO₄.5H₂O                         2.5 mg/L

CoSO₄.7H₂O                         2.8 mg/L

KI                                  83 mg/L

 

100хN6 vitamins

thiamine hydrochloride VB1        100mg /L

pyridoxine hydrochloride VB6      50mg/L

nicotinic acid                    50 mg/L

Gly                            200g/L

 

100хB5 vitamins

thiamine hydrochloride VB1        1g/L

pyridoxine hydrochloride VB6      100 mg/L

nicotinic acid                    100 mg/L

myo-inositol                    10 g/L

 

100х modified MS vitamins

thiamine hydrochloride VB1        100mg /L

pyridoxine hydrochloride VB6      50mg/L

nicotinic acid                    50 mg/L

Gly                            200mg/L

myo-inositol                    10g/L

 

100хCC vitamins

thiamine hydrochloride VB1        850mg /L

pyridoxine hydrochloride VB6      100mg/L

nicotinic acid                    600 mg/L

Gly                            200g/L

myo-inositol                    9g/L

 

 

 

Composition	MS (mg/l)a	N6 (mg/l)b	L3 (mg/l)	Dl (mg/l)	B5 (mg/l)c	AA (mg/l)d
major
KNO3	1,900	2,830	1,900	2,900	2,500	2,950 (KCl)
NH4NO3	1,650		1,700
(NH4)2SO4		463		450	134
KH2PO4	170	400	170	400	150	170 (Na2HPO4)
MgSO4·7H2O	370	185	370	185	250	500
CaCl2·2H2O	440	166	400	170	150	150
Micronutrient
FeSO4·7H2O	27.85	27.85	41.8	55.9	27.8	27.8
Na2EDTA	37.25	37.25	55.9	74.5	37.3	37.3
MnSO4·4H2O	22.3	4.4	100	100	10	10
ZnSO4·7H2O	8.6	1.5	20	20	2	2
H3BO3	6.2	1.6	30	30	3	3
KI	0.83	0.8	7.5	7.5	0.75	0.75
CuSO4·5H2O	0.025		0.25	0.25	0.025	0.025
Na2MoO4·2H2O	0.25		2.5	2.5	0.25	0.25
CoCl2·6H2O	0.025		0.25	0.25	0.025	0.025
Organic
Glycine	2.0	2.0	2.0	2.0		2.0
Thiamine	0.4	1.0	1.0	1.0	10.0	10.0
Pyridoxine	0.5	0.5	1.0	1.0	1.0	1.0
Nicotinic acid	0.5	0.5	1.0	1.0	1.0	1.0
i-Inositol	100	100	100	100	100	100
l-Glutamine						876
l-Asparagine						216
l-Arginine						176

 

a Murashige and Skoog 1962

b Chu et al. 1975

c Gamborg et al. 1968

d Toriyama and Hinata 1985

 Plant Cell Rep (2005) 23:540–547

籼稻转化诱导愈伤培养基L3+2.5 2,4-D+500毫克脯氨酸+500谷氨酰胺+3%的麦芽糖+0.25%胶

分化D+500毫克脯氨酸+500谷氨酰胺+800毫克CH+2毫克 BA+2毫克KT+0.2毫克NAA+0.2毫克IAA+3%的麦芽糖+0.3%胶

Sigma  E6760 The theoretical iron content is 15.2%,亚铁离子的工作浓度为0.1mM, E6760的分子量为367.1,含水量为9%,配置100倍的铁盐1000毫升需要E6760  4.03克.

 

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