Chromosome-level genome assembly of Ophiorrhiza pumilareveals the evolution of camptothecin biosynthesis

第一作者：Amit Rai

第一单位：千叶大学

通讯作者：Mami Yamazaki

 Abstract 

背景回顾：Plant genomes remain highly fragmented and are often characterized by hundreds to thousands of assembly gaps. 

主要研究：Here, we report chromosome-level reference and phased genome assembly of Ophiorrhiza pumila, a camptothecin-producing medicinal plant, through an ordered multi-scaffolding and experimental validation approach.

结果1-组装质量：With 21 assembly gaps and a contig N50 of 18.49 Mb, Ophiorrhiza genome is one of the most complete plant genomes assembled to date.

结果2-MIA代谢：We also report 273 nitrogen-containing metabolites, including diverse monoterpene indole alkaloids (MIAs).

结果3-MIA起源：A comparative genomics approach identifies strictosidine biogenesis as the origin of MIA evolution. The emergence of strictosidine biosynthesis-catalyzing enzymes precede downstream enzymes’ evolution post γ whole-genome triplication, which occurred approximately 110 Mya in O. pumila, and before the whole-genome duplication in Camptotheca acuminata identified here.

结论：Combining comparative genome analysis, multi-omics analysis, and metabolic gene-cluster analysis, we propose a working model for MIA evolution, and a pangenome for MIA biosynthesis, which will help in establishing a sustainable supply of camptothecin.

 通讯作者 

**Mami Yamazaki**

doi: https://doi.org/10.1038/s41467-020-20508-2

Journal: Nature Communications

Published date: Jan 15, 2021