A GRF–GIF chimeric protein improves the regeneration efficiency of transgenic plants

第一作者：Juan M. Debernardi

第一单位：加利福尼亚大学

通讯作者：Jorge Dubcovsky

 Abstract 

研究背景：The potential of genome editing to improve the agronomic performance of crops is often limited by low plant regeneration efficiencies and few transformable genotypes.

主要研究：Here, we show that expression of a fusion protein combining wheat GROWTH-REGULATING FACTOR 4 (GRF4) and its cofactor GRF-INTERACTING FACTOR 1 (GIF1) substantially increases the efficiency and speed of regeneration in wheat, triticale and rice and increases the number of transformable wheat genotypes.

结果1-GRF4–GIF1植株无不良影响：GRF4–GIF1 transgenic plants were fertile and without obvious developmental defects.

结果2-GRF4–GIF1植株高效诱导植株再生：Moreover, GRF4–GIF1 induced efficient wheat regeneration in the absence of exogenous cytokinins, which facilitates selection of transgenic plants without selectable markers.

结果3-GRF4–GIF1系统的应用拓展：We also combined GRF4–GIF1 with CRISPR–Cas9 genome editing and generated 30 edited wheat plants with disruptions in the gene Q (AP2L-A5).

结果4-GRF4–GIF1系统同样适用双子叶植物：Finally, we show that a dicot GRF–GIF chimera improves regeneration efficiency in citrus, suggesting that this strategy can be applied to dicot crops.

 通讯作者 

doi: 10.1038/s41587-020-0703-0

Journal: Nature Biotechnology

Published date: Oct 12, 2020