Genome‐wide functional analysis of hot pepper immune receptors reveals an autonomous NLR clade in seed plants

第一作者：Hye‐Young Lee

第一单位：首尔国立大学

通讯作者：Doil Choi

 Abstract 

背景介绍：Plants possess hundreds of intracellular immune receptors encoding nucleotide‐binding domain leucine‐rich repeat (NLR) proteins. Full‐length NLRs or a specific domain of NLRs often induce plant cell death in the absence of pathogen infection.

主要研究：In this study we used genome‐wide transient expression analysis to identify a group of NLRs (ANLs; ancient and autonomous NLRs) carrying autoactive coiled‐coil (CCA) domains in pepper (Capsicum annuum).

结果1-CCA介导植物细胞死亡：CCA‐mediated cell death mimics hypersensitive cell death triggered by the interaction between NLRs and pathogen effectors.

结果2-CCA必要结构α1螺旋：Sequence alignment and mutagenesis analyses revealed that the intact α1 helix of CCAs is critical for both CCA‐ and ANL‐mediated cell death.

结果3-ANLs是singleton：Cell death induced by CCAs does not require NRG1/ADR1 or NRC type helper NLRs, suggesting ANLs may function as singleton NLRs. We also found that CCAs localize to the plasma membrane, as demonstrated for Arabidopsis singleton NLR ZAR1.

结果4-进化分析：Extended studies revealed that autoactive CCAs are well conserved in other Solanaceae plants as well as in rice, a monocot plant. Further phylogenetic analyses revealed that ANLs are present in all tested seed plants (spermatophytes).

结论：Our study not only uncovers the autonomous NLR clade in plants but also provides powerful resources for dissecting the underlying molecular mechanism of NLR‐mediated cell death in plants.

doi: 10.1111/nph.16878

Journal: New Phytologist

Published online: Nov 16, 2020