LOCUS       KU899088                8282 bp    DNA     circular SYN 11-MAY-2016DEFINITION  Expression vector NLS-NgAgo-pcDNA3.1, complete sequence.

ACCESSION   KU899088VERSION     KU899088.1  GI:1026284473

KEYWORDS    .

SOURCE      Expression vector

NLS-NgAgo-pcDNA3.1  

ORGANISM  Expression vector NLS-NgAgo-pcDNA3.1other sequences; artificial sequences; vectors.

REFERENCE   1  (bases 1 to 8282)  

AUTHORS   Gao,F., Shen,X.Z., Jiang,F., Wu,Y. and Han,C.  

TITLE     DNA-guided genome editing using the Natronobacterium gregoryi            Argonaute  

JOURNAL   Nat. Biotechnol. (2016) In press  

PUBMED   27136078  REMARK    Publication Status: Available-Online prior to print

REFERENCE   2  (bases 1 to 8282)

AUTHORS   Gao,F.  

TITLE     Direct Submission  

JOURNAL   Submitted (11-MAR-2016)

Department of Bioscience and            Bioengineering, Hebei University of Science and Technology, Yu            Xiang Street 26, Shijiazhuang, Hebei 050018, China

COMMENT     ##Assembly-Data-START##            Sequencing Technology :: Sanger dideoxy sequencing            ##Assembly-Data-END##

FEATURES             Location/Qualifiers     source          1..8282                     /organism="Expression vector NLS-NgAgo-pcDNA3.1"                     /mol_type="other DNA"                     /db_xref="taxon:1844592"                     /country="China"                     /note="backbone derived from pcDNA3.1/Hygro(+); NgAgo                     derived from Natronobacterium gregoryi SP2"CDS             923..3613                     /note="NgAgo; includes nuclear localization sequence"                     /codon_start=1                     /transl_table=11                     /product="argonaute"                     /protein_id="ANC90310.1"                     /db_xref="GI:1026284474"                     /translation="MVPKKKRKVATVIDLDSTTTADELTSGHTYDISVTLTGVYDNTD                     EQHPRMSLAFEQDNGERRYITLWKNTTPKDVFTYDYATGSTYIFTNIDYEVKDGYENL                     TATYQTTVENATAQEVGTTDEDETFAGGEPLDHHLDDALNETPDDAETESDSGHVMTS                     FASRDQLPEWTLHTYTLTATDGAKTDTEYARRTLAYTVRQELYTDHDAAPVATDGLML                     LTPEPLGETPLDLDCGVRVEADETRTLDYTTAKDRLLARELVEEGLKRSLWDDYLVRG                     IDEVLSKEPVLTCDEFDLHERYDLSVEVGHSGRAYLHINFRHRFVPKLTLADIDDDNI                     YPGLRVKTTYRPRRGHIVWGLRDECATDSLNTLGNQSVVAYHRNNQTPINTDLLDAIE                     AADRRVVETRRQGHGDDAVSFPQELLAVEPNTHQIKQFASDGFHQQARSKTRLSASRC                     SEKAQAFAERLDPVRLNGSTVEFSSEFFTGNNEQQLRLLYENGESVLTFRDGARGAHP                     DETFSKGIVNPPESFEVAVVLPEQQADTCKAQWDTMADLLNQAGAPPTRSETVQYDAF                     SSPESISLNVAGAIDPSEVDAAFVVLPPDQEGFADLASPTETYDELKKALANMGIYSQ                     MAYFDRFRDAKIFYTRNVALGLLAAAGGVAFTTEHAMPGDADMFIGIDVSRSYPEDGA                     SGQINIAATATAVYKDGTILGHSSTRPQLGEKLQSTDVRDIMKNAILGYQQVTGESPT                     HIVIHRDGFMNEDLDPATEFLNEQGVEYDIVEIRKQPQTRLLAVSDVQYDTPVKSIAA                     INQNEPRATVATFGAPEYLATRDGGGLPRPIQIERVAGETDIETLTRQVYLLSQSHIQ                     VHNSTARLPITTAYADQASTHATKGYLVQTGAFESNVGFL"ORIGIN              1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg   61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg  121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc  181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt  241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata  301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc  361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc  421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt  481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt  541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca  601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg  661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc  721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg  781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca  841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc  901 gtttaaactt aagcttgcca ccatggtgcc aaaaaagaag agaaaggtag ccacagtgat  961 tgacctcgat tcgaccacca ccgcagacga actgacatcg ggacacacgt acgacatctc 1021 agtcacgctc accggtgtct acgataacac cgacgagcag catcctcgca tgtctctcgc 1081 attcgagcag gacaacggcg agcggcgtta cattaccctg tggaagaaca cgacacccaa 1141 ggatgtcttt acatacgact acgccacggg ctcgacgtac atcttcacta acatcgacta 1201 cgaagtgaag gacggctacg agaatctgac tgcaacatac cagacgaccg tcgagaacgc 1261 taccgctcag gaagtcggga cgactgacga ggacgaaacg ttcgcgggcg gcgagccgct 1321 cgaccatcac ttggacgacg cgctcaatga gacgccagac gacgcggaga cagagagcga 1381 ctcaggccat gtgatgacct cgttcgcctc ccgcgaccaa ctccctgagt ggacgctgca 1441 tacgtatacg ctaacagcca cagacggcgc aaagacggac acggagtacg cgcgacgaac 1501 cctcgcatac acggtacggc aggaactcta taccgaccat gatgcggctc cggttgcaac 1561 tgacgggcta atgcttctca cgccagagcc gctcggcgag accccgcttg acctcgattg 1621 cggtgtccgg gtcgaggcgg acgagactcg gacactcgat tacaccacgg ccaaagaccg 1681 gttactcgcc cgcgaactcg tcgaagaggg gctcaaacgc tccctctggg atgactacct 1741 cgttcgcggc atcgatgaag tcctctcaaa ggagcctgtg ctgacttgcg atgagttcga 1801 cctacatgag cggtatgacc tctctgtcga agtcggtcac agtgggcggg cgtaccttca 1861 catcaacttc cgccaccggt tcgtaccgaa gctgacgctc gcagacatcg atgatgacaa 1921 catctatcct gggctccggg tgaagacgac gtatcgcccc cggcgaggac atatcgtctg 1981 gggtctgcgg gacgagtgcg ccaccgactc gctcaacacg ctgggaaacc agtccgtcgt 2041 tgcataccac cgcaacaatc agacacctat taacactgac ctcctcgacg ctatcgaggc 2101 cgctgaccgg cgagtcgtcg aaacccgacg tcaagggcac ggcgatgatg ctgtctcatt 2161 cccccaagaa ctgcttgcgg tcgaaccgaa tacgcaccaa attaagcagt tcgcctccga 2221 cggattccac caacaggccc gctcaaagac gcgtctctcg gcctcccgct gcagcgagaa 2281 agcgcaagcg ttcgccgagc ggcttgaccc ggtgcgtctc aatgggtcca cggtagagtt 2341 ctcctcggag tttttcaccg ggaacaacga gcagcaactg cgcctcctct acgagaacgg 2401 tgagtcggtt ctgacgttcc gcgacggggc gcgtggtgcg caccccgacg agacattctc 2461 gaaaggtatc gtcaatccac cagagtcgtt cgaggtggcc gtagtactgc ccgagcagca 2521 ggcagatacc tgcaaagcgc agtgggacac gatggctgac ctcctcaacc aagctggcgc 2581 gccaccgaca cggagcgaga ccgtccaata tgatgcgttc tcctcgccag agagcatcag 2641 cctcaatgtg gctggagcca tcgaccctag cgaggtagac gcggcattcg tcgtactgcc 2701 gccggaccaa gaaggattcg cagacctcgc cagtccgaca gagacgtacg acgagctgaa 2761 gaaggcgctt gccaacatgg gcatttacag ccagatggcg tacttcgacc ggttccgcga 2821 cgcgaaaata ttctatactc gtaacgtggc actcgggctg ctggcagccg ctggcggcgt 2881 cgcattcaca accgaacatg cgatgcctgg ggacgcagat atgttcattg ggattgatgt 2941 ctctcggagc taccccgagg acggtgccag cggccagata aacattgccg cgacggcgac 3001 cgccgtctac aaggatggaa ctatcctcgg ccactcgtcc acccgaccgc agctcgggga 3061 gaaactacag tcgacggatg ttcgtgacat tatgaagaat gccatcctcg gctaccagca 3121 ggtgaccggt gagtcgccga cccatatcgt catccaccgt gatggcttca tgaacgaaga 3181 cctcgacccc gccacggaat tcctcaacga acaaggcgtc gagtacgaca tcgtcgaaat 3241 ccgcaagcag ccccagacac gcctgctggc agtctccgat gtgcagtacg atacgcctgt 3301 gaagagcatc gccgctatca accagaacga gccacgggca acggtcgcca ccttcggcgc 3361 acccgaatac ttagcgacac gcgatggagg cggccttccc cgcccaatcc aaattgaacg 3421 agtcgccggc gaaaccgaca tcgagacgct cactcgccaa gtctatctgc tctcccagtc 3481 gcatatccag gtccataact cgactgcgcg cctacccatc accaccgcat acgccgacca 3541 ggcaagtact cacgcgacca agggttacct cgtccagacc ggagcgttcg agtctaatgt 3601 cggattcctc tgaggatcca ctagtccagt gtggtggaat tctgcagata tccagcacag 3661 tggcggccgc tcgagtctag agggcccgtt taaacccgct gatcagcctc gactgtgcct 3721 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt 3781 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg 3841 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac 3901 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc 3961 tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg 4021 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct 4081 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg 4141 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag 4201 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg 4261 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc 4321 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat 4381 gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt 4441 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt 4501 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc 4561 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc 4621 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg 4681 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc 4741 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg 4801 tgatgaaaaa gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg 4861 acagcgtctc cgacctgatg cagctctcgg agggcgaaga atctcgtgct ttcagcttcg 4921 atgtaggagg gcgtggatat gtcctgcggg taaatagctg cgccgatggt ttctacaaag 4981 atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca 5041 ttggggaatt cagcgagagc ctgacctatt gcatctcccg ccgtgcacag ggtgtcacgt 5101 tgcaagacct gcctgaaacc gaactgcccg ctgttctgca gccggtcgcg gaggccatgg 5161 atgcgatcgc tgcggccgat cttagccaga cgagcgggtt cggcccattc ggaccgcaag 5221 gaatcggtca atacactaca tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt 5281 atcactggca aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg 5341 agctgatgct ttgggccgag gactgccccg aagtccggca cctcgtgcac gcggatttcg 5401 gctccaacaa tgtcctgacg gacaatggcc gcataacagc ggtcattgac tggagcgagg 5461 cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt cttctggagg ccgtggttgg 5521 cttgtatgga gcagcagacg cgctacttcg agcggaggca tccggagctt gcaggatcgc 5581 cgcggctccg ggcgtatatg ctccgcattg gtcttgacca actctatcag agcttggttg 5641 acggcaattt cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg 5701 gagccgggac tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc tggaccgatg 5761 gctgtgtaga agtactcgcc gatagtggaa accgacgccc cagcactcgt ccgagggcaa 5821 aggaatagca cgtgctacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct 5881 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg 5941 agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata 6001 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca 6061 aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt 6121 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca 6181 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat 6241 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt 6301 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct 6361 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 6421 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 6481 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 6541 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 6601 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 6661 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 6721 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 6781 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 6841 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 6901 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 6961 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 7021 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtttt tttgtttgca 7081 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 7141 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa 7201 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta 7261 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag 7321 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga 7381 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac 7441 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc 7501 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta 7561 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac 7621 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat 7681 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa 7741 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg 7801 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag 7861 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc 7921 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct 7981 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat 8041 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg 8101 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc 8161 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 8221 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 8281 tc//