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今天早晨看到李老师的博文,感到很惊讶:诺贝尔奖的诱导多能干细胞的新发展-一个可能的警告!http://blog.sciencenet.cn/home.php?mod=space&uid=847277&do=blog&id=726543
看来这个诺贝尔奖发的太着急了,没有经过多少时间的考验。
Reprogramming in vivo produces teratomas and iPS cells with totipotency features.
Tumour Suppression Group, Spanish National Cancer Research Centre (CNIO), Madrid E-28029, Spain.
Reprogramming of adult cells to generate induced pluripotent stem cells (iPS cells) has opened new therapeutic opportunities; however, little is known about the possibility of in vivo reprogramming within tissues. Here we show that transitory induction of the four factors Oct4, Sox2, Klf4 and c-Myc in mice results in teratomas emerging from multiple organs, implying that full reprogramming can occur in vivo. Analyses of the stomach, intestine, pancreas and kidney reveal groups of dedifferentiated cells that express the pluripotency marker NANOG, indicative of in situ reprogramming. By bone marrow transplantation, we demonstrate that haematopoietic cells can also be reprogrammed in vivo. Notably, reprogrammable mice present circulating iPS cells in the blood and, at the transcriptome level, these in vivo generated iPS cells are closer to embryonic stem cells (ES cells) than standard in vitro generated iPS cells. Moreover, in vivo iPS cells efficiently contribute to the trophectoderm lineage, suggesting that they achieve a more plastic or primitive state than ES cells. Finally, intraperitoneal injection of in vivo iPS cells generates embryo-like structures that express embryonic and extraembryonic markers. We conclude that reprogramming in vivo is feasible and confers totipotency features absent in standard iPS or ES cells. These discoveries could be relevant for future applications of reprogramming in regenerative medicine.
Nature Publishing Group http://www.nature.com/nature/journal/vaop/ncurrent/full/nature12586.html
许教授 您好:
我读到您的博客,诺贝尔奖的诱导多能干细胞的新发展-一个可能的警告!
谢谢郑总,你的分析结果很重要,我加上这些分析数据,供科研人员参考。
许培扬
检索策略:
(("teratoma"[MeSH Terms] OR "teratoma"[All Fields] OR "teratomas"[All Fields]) AND ("induced pluripotent stem cells"[MeSH Terms] OR ("induced"[All Fields] AND "pluripotent"[All Fields] AND "stem"[All Fields] AND "cells"[All Fields]) OR "induced pluripotent stem cells"[All Fields] OR ("ips"[All Fields] AND "cells"[All Fields]) OR "ips cells"[All Fields])) AND "teratoma"[mesh]
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Database: | ||||
PubMed | ||||
User query: | ||||
teratomas and iPS cells AND ("teratoma"[mesh]) |
检索分析平台 http://www.pubmedplus.cn/
检索时间 2013年9月21日 8时
检索结果 PUBMED数据库检索到相关文献178篇
信息分析结果如下:
研究主题领域:
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